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    OPTIMIZE, STANDARDIZE, AUTOMATE AND DOCUMENT YOUR DECALCIFICATION PROTOCOLS

    The DecalMATE system is a new tool that enables histotechs to “tailor” their fixation/decalcification process to the specific characteristics of their bone tissue specimens.


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    SIMPLE, AUTOMATIC, SAFE.

    For the first time, automatic protocols for the fixation/decalcification of bone tissues are available to modern laboratories to improve the diagnostic quality and the consistency of results with a faster turnaround time. The intuitive, user-friendly software guides the operator on the touch screen control terminal. The unit consists of a resistance-heating, temperature controlled process cavity, made of high chemical resistant techno polymers, with a built-in magnetic stirring device for temperature and concentration homogeneity.



    After placing the rack in the cavity and pressing start, the unit will automatically:

    1. Transfer the fixative from the tank to the process cavity
    2. Bring the fixative to the selected temperature for the preset time
    3. Drain, after time expires, the fixative solution back to the tank
    4. Transfer the decalcifying solution to the cavity and heat it for the selected temperature and time
    5. Drain the solution back to the tank after completion

    Two flushing/rinsing steps are automatically carried out between the two phases. An alarm buzzer advises the operator about the completion of the steps.

    WHICH RACK FOR WHICH SPECIMENS?

    Simply position the cassettes in the rack. Position the rack in the cavity and close the cover. Select your protocol and press START. After the decalcification, check the status of the bone.


    STANDARD

    30 cassettes

    For specimens of 30x25x5 mm dimensions


    MEGA

    16 cassettes

    For specimens of 30x25x10 mm dimensions


    SUPER MEGA

    6 cassettes

    For specimens of 70x50x15 mm dimensions





    THIS IS HOW DECALCIFICATION SHOULD BE

    All major parameters influencing all these important pre-analytical steps can be controlled, optimized, automated and documented.

    TEMPERATURE

    Fixation and decalcification temperature can be set between 20-55°C for an optimization of the process.


    MAGNETIC STIRRING (AGITATION)

    The calcium ions that have been removed can saturate the solution around the specimen. Continuous stirring assures a consistent flow of fresh decalcifier onto the bone surface, accelerating the decalcification process:



    SIZE OF SPECIMENS

    Kullanıcının gereksinimlerine uyacak şekilde üç farklı raf tipi mevcuttur.

    REAGENTS

    Proses devrelerinde yer alan tüm ürün yüzeyleri aşağıdaki reaktiflerle uyumludur:

    FIXATIVES

    DECALCIFICATION SOLUTIONS
    Finefix EDTA 20% at max 50#C
    Formalin Formic acid 20% at max 50°C
    Acetic acid 20% at max 50°C
    Hydrochloric acid 10% at max 37°C
    Nitric acid 10% at max 37°C
    Trichloroacetic acid 10% at max 37°C

    DECALCIFICATION OF BONE MARROW

    OPTIMIZING DECALCIFICATION FOR MOLECULAR STUDIES

    These concluded that there was a significant decrease in both RNA and DNA yield and integrity with strong acids (hydrochloric, nitric) versus 12,5-14% of EDTA and formic acid. They also concluded that the preferred decalcifying agents to optimize the quantity and quality of recovery of nucleic acids from bone biopsies specimens are those that contain a sole agent or a combination of either 14% of EDTA or formic acid.

    MOL-DECALCIFIER

    DECALCIFIER SOLUTION FOR OPTIMAL MOLECULAR STUDIES

    For a faster decalcification time, the process with EDTA is best carried out at a pH value of 7.2-7.4. Milestone has developed an innovative, pure EDTA solution, based on a stoichiometric mixture of bibasic and tribasic EDTA to reach this pH value without the addition of an acid/base buffer. The combination of an increased temperature, stirring and the MoL-DECALCIFIER, during the decalcification phase allows fixation, decalcification and processing of bone marrows within 48 hours.
    FIXATION DECAL. TEMP. TOTAL TIME
    Formalin 10% MoL-DECALCIFIER °C Fixation + Decal.
    1h 30’ 16h 30’ 37 18h
    1h 4h 50 5h
    Formalin 10% Formic 10% °C Fixation + Decal.
    1h 30’ 1h 30’ 37 3h
    1h 1h 50 2h
    DecalMATE protocols for bone marrows (Ø 1.8mm)