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Bio Basic Canada Inc. is a privately owned dynamic biotechnology company. The company was founded in 1990 in Toronto, Canada. From 1990 to 1995, Bio Basic Canada Inc.’s primary focus has been in the field of biochemicals. Starting in 1995, Bio Basic Canada Inc. has begun manufacturing various Life Science Products. Throughout the past years, the company has developed rapidly and served as a one-stop-shop to our customers in the life sciences field. To date, Bio Basic Canada Inc. has approximately 600 employees, seven laboratories, three factory buildings, 40 international distributors and over 10,000 customers worldwide.
Bio Basic Canada Inc. is committed to innovation and excellence. In an effort to achieve this, Bio Basic Canada Inc. has continuously directed efforts in R & D and improving the quality of our products. Bio Basic Canada Inc. is proud to offer a breath of products and services to customers worldwide. Several of our product lines such as, Molecular Biology Kits, Biochemicals, Synthetic Genes and Synthetic Oligos have already shown dominant market presence and have been widely recognized by customers worldwide.
Although there are thousands of companies in the world selling life science products, Bio Basic Canada Inc. distinguishes itself from our other competitors in the following five features
(1) Manufacturer. We have ten product lines, eight of which are manufactured within our state-of-the-art facility.
(2) Wide range of products.
(3) Lowest price for all product lines.
(4) Strict quality control.
(5) Ambitious. Bio Basic Canada Inc. has a dream to become the world leader in the field of life science products. A fast growing history indicates this dream is well on its way to being true!
In this year’s catalogue, Bio Basic Canada Inc. is delighted to introduce the following new products to serve your needs: complete range of molecular biology kits, protein-chemistry related productsand much more……………….
Bio Basic Canada Inc.’s Commitment:
To Offer Researchers Highest Quality Products At Most Affordable Prices. |
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PRODUCTS
Six Comprehensive Product Lines:
Chemicals including Inorganic Salts, Buffers, Biological Stains, Antibiotics, Amino
Acids, Nucleosides and Nucleotide, PCR Related Products, Plant growth regulators
Molecular Biology Kits
PCR related products & RNA, DNA Modifying enzymes
DNA, RNA, Protein Markers and Vectors
Culture Medium
Labware
Five High Quality Services:
Custom Oligos
Gene Synthesis
Peptide Synthesis
DNA Sequencing
Protein Purification/Expression |
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BIOCHEMICALS
We here at Bio Basic Canada Inc. sell a large variety of biochemical’s, organic and inorganic chemicals and related products. With over 70 distributors worldwide and success of over 14 years, Bio Basic Canada Inc. has become a world leader in supplying biochemical’s, Bio Basic Canada Inc. strives to offer HIGHEST Quality and LOWEST Prices to our customers. At Bio Basic Canada Inc., you will find the products to help you reach your ultimate goals.
Bio Basic Canada Inc's products include antibiotics, nucleotides, proteins, peptides, polyamino acids, buffers, lipids, and carbohydrates all applicable for fundamental research. Our staff assures that the biochemical’s are of highest purity and top quality. We strive for excellence and take pride in delivering to our clients and assuring their happiness. Our biochemicals are stored in a state of the art warehouse where optimal conditions are set for the biochemicals so they work for your experimental needs.
In addition to biochemicals, Bio Basic Canada Inc. also carries a tremendous variety of other reagents which makes us versatile for any clients needs. These products range from Markers, to Primers, to Mediums, to Protein Related products, to Labwares, and Complete Biochemical Kits.
Please also ask about our specialty products, including Agarose, RNaseA, dNTP, Proteinase K and much more. |
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MOLECULAR BIOLOGY KITS
Bio Basic Canada Inc. supplies a remarkable abundance line of molecular biology kits. We have three main types of kits: Plasmid Miniprep kits, Gel Extraction kits and PCR Products purification kits. In addition, Bio Basic Canada Inc, is proud to offer a complete line of Genomic DNA isolation kits and RNA isolation kits. On top of that we have launched a wide array of more and newer kits including Rapid Animal Total RNA Extraction Kit, Rapid Plant Genomic DNA extraction Kit, Tiny Sample DNA/RNA Recovery Kit, Clean-up Mini Kit and even a 5 Minute Spin Column Plasmid-Mini Preps Kit.
We take great care in ensuring the highest quality in our kits and their exceptional quality will allow you to complete your objectives and carry out your experiments and goals in the laboratory, research lab, university classroom, and institute.
Kits Key Features Include
(1) Direct Manufacturer
Bio Basic Canada Inc. manufactures every component of the molecular biology kits within our own facility
(2) Wide Range of Products
Bio Basic Canada Inc. provides mini-preps, medi-preps, maxi-preps and all 96-well format plate kits
(3) Strict Quality Control
Each lot of kit is strictly examined for quality assurance before reaching customers
(4) Lowest Prices
Please inquire about the bulk prices |
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PCR RELATED PRODUCTS
When using and dealing with polymerase chain reaction experiments, it is vital that the enzymes and reagents are as optimal and close to perfect as can be. We here at Bio Basic Canada Inc.’s have the highest purity enzymes and substrates for your experiments and at a fantastic rate you can’t find anywhere else.
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Enzymes |
Tsg DNA Polymerase |
| Tsg DNA Polymerase High Purity |
| Tsg Plus DNA Polymerase |
| Taq DNA Polymerase |
| Taq DNA Polymerase High Purity |
| Taq Plus DNA Polymerase |
| Pfu DNA Polymerase |
| AMV Reverse Transcriptase |
| M-Mulv Reverse Transcriptase |
| T4 DNA Ligase |
| T4 DNA Ligase with PEG |
| Uracil DNA Glycosylase |
When you order any enzyme from Bio Basic Canada Inc. you are getting a high quality product that will conduct its specified purpose and perform on the PCR experiment.
All are enzymes are quality control checked and made sure they are as pure as can be and are ready to allow you to conduct your experiments and get the results you hoped for. Our rates allow us to have a competitive edge over against other suppliers and Bio Basic Canada Inc is number one when you inquire about an enzyme, to a peptide, to a gene, to a biochemical. |
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DNA, RNA, PROTEIN MARKERS
Bio Basic Canada Inc. has a complete line of DNA, RNA and protein markers to serve an increasingly sophisticated market that demands premium quality. Bio Basic Canada Inc. offers the flexibility to meet your needs as some of the DNA/RNA markers are less than 20 base pairs and up to 10,000 base pairs. Bio Basic Canada Inc’s markers are also highly competitive in prices. Please inquire Bulk prices.
In addition to DNA, RNA and protein markers, Bio Basic Canada Inc offers complete series of vector DNA from pUC57, pUC18, pCU19, pBR322 to Lamda phage DNA in ug, mg and/or grams quantity. Bio Basic Canada Inc. is a one-stop shop for all your research needs.
Bio Basic Canada Inc. strives for excellence. Our laboratories verify and make assured that the markers going to customer are experimental ready to deliver and execute with excellence. |
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PROTEIN RELATED PRODUCTS
When dealing with proteins there can be complex requirements to conduct the experiment and Bio Basic Canada Inc. has the products you need. Whether it’s an inhibitor cocktail, to a protein solubilizer, to western blot staining, we here at Bio Basic Canada Inc. have your necessary items.
All of are products we send are made assure they are of the highest purity and will execute and deliver in performance in your laboratory. Bio Basic Canada Inc. takes great honor in providing you with products and we do strict quality control protocols on all of our products before they leave our facility.
Sometimes proteins and items are temperature sensitive and require to be kept in optimal cold conditions. We take care of that worry and concern by implementing our ice cold bio basic pack for you in the shipment. We work immediately on your order and keep you up to date with any issues you may have.
From resins, to kits, to staining and blotting, we have the protein repertoire for you to be successful in your laboratory, research, educational setting, to institutes. |
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CULTURE MEDIUMS
Bio Basic Canada Inc. has been serving customers a great variety of culture medium including agars, animal/plant peptones, yeast extract for the growth of microorganisms in many diverse applications such as microbiology and fermentation.
Bio Basic Canada Inc. also provides high quality and extended series of Broth from LB, TY, SOC, HYLB, A Medium, H Medium to NZYM Broth and much more……..
Bio Basic Canada Inc. draws on its strong technical and manufacturing expertise to supply consistently high quality raw materials to the world.
Our mediums we use have the highest nutrient and purity for maximal results. We have over 100 mediums to choose from. All mediums are stored at the correct storage conditions and also are pure in their form.
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TYPE |
UTILIZES |
| Nutrient media |
-Carbon sources
-Water
-Various salts
-Source of amino acids and nitrogen |
| Minimal media |
-Carbon source
-Sugars
-Mineral elements
-Water |
| Selective media |
-Antibiotics
-Amino acids |
| Differential media |
-Indicators |
| Transport media |
-Buffer
-Salt |
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LABWARES
Every science laboratory to educational laboratories to research faculties relies upon labware ranging from PCR tubes, plastic bottles, storage beakers and even to brushes. Bio Basic Canada Inc. offers a wide range of labware including tubes, tips, containers, bottles, transport and storage, as well as a complete range of cryogenic tubes, and storage boxes. Our versatile selection leaves you the ability to perform any experiment you need to do. Our different categories of labwares also allow you to make your laboratory legit and experiment ready.
All are items are kept in great tip top shape and are assured they are packaged accordingly to come to you as they are meant to be; perfect. Bio Basic Canada Inc. is proud to be the DIRECT manufacturer for Bio Basic Canada Inc.'s labwares. Almost all plasticwares are manufactured within Bio Basic Canada Inc's state-of-the-art 500,000 sq feet manufacture facility. Bio Basic Canada Inc. labwares have been widely distributed among universities, hospitals, industrial and research laboratories.
If you have an item that needs to be kept cold, we even have our own little bio basic ice bag for you to keep your essentials at the optimal temperature. Have a time sensitive experiment to conduct and want to record reactions? we have a timer that can do that for you. Other items such as racks, test tubes, weighing paper, spatulas, scoops, labels, buckets, trays, and gloves are also what we are able to supply you with and are laboratory essentials.
All are products are Quality Control tested before we ship them out. Every product you order from Bio Basic Canada Inc whether its from biochemical’s, to peptides, to genes, even our lab wares are made assured they are free of contamination, cracks, have all items, quantities, and are ready to come into your laboratory ready to hit the ground running and meet your needs! |
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DNA SEQUENCING
The Highest Standard in DNA Sequencing Services
We currently offer the following DNA Sequencing-related services:
Large-scale DNA sequencing (>100 samples)
Small-scale DNA sequencing (<100 samples)
Plasmid DNA preps from glycerol stocks in advance of sequencing
PCR Products Sequencing
Key Features
· Multiple of 3730XL sequencers stand by
· Sequence up to 800bp per reaction, more than 90% successful rate
· Special prices on large projects and contracts
· 24hr to 48 hour turnaround
· Incoming sample QC
· Fluorescent dye-terminator sequencing
· >500bp Q20 read lengths typical
· Universal primers provided at no additional charge (complete list)
· Template preparation and primer synthesis available
Sample Requirement
We accept plasmid DNA (100ng/ul, 20ul), bacteria, PCR products (crude or purified).
Note: Please specify vector name and insert length and resistance.
PCR (crude): concentration > 30ng/ul, volume >50ul
PCR (purified): concentration > 30ng/ul, volume > 20ul
Note: PCR sample, please indicate the length of insert (bp)
Please indicate the concentration of the primer enclosed with sample.
The following primers are provided free of charge: M13+, M13-, T7, T3, T7 Terminator, SP6, pGEX5’, pGEX3', BGH rev.
Samples are permanently destroyed after two months. |
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GENE SYNTHESIS
Largest Professional in Gene Synthesis Field and now approved for ISO9001:2000
With highly experienced staff biologists in the Department of Gene Synthesis, Bio Basic Canada Inc. is one of the largest professional organizations in the field for gene synthesis. For the past seven years, Bio Basic Canada Inc., as a silent OEM provider, has synthesized millions of base pairs of genes to worldwide customers throughout our agents. Now, Bio Basic Canada Inc. is delighted to offer high quality gene synthesis services DIRECTLY to end users like you. Benefiting from our own Oligo house and DNA sequencing facility, Bio Basic Canada Inc. offers gene synthesis at the most affordable prices. So far, we have satisfied and impressed our customers from leading biotechnology, pharmaceutical industries to famous academic institutions. |
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OLIGO SYNTHESIS
One of the Largest Professionals in Oligo Synthesis
Features:
. Highest Purity at Lowest Price
. Specializing in HAP* (High Affinity Purification) and PAGE Purification
. Worldwide Services
. High Capacity: 5000 primers/day
. One of the Largest and the Most Experienced Suppliers
. Fast Services: Synthesis Completed within 24 or 36 Hours (for HAP* or PAGE)
. All modified base oligos are purified by HPLC (WAVE system)
. Highest QC and Quality Guaranteed
. Wide Range Product Lines
High Affinity Purification (HAP) is a patented, novel purification method for custom oligos developed by Bio Basic Canada Inc. DMT-ON-Oligo in the crude oligo mixture is first selectively absorbed on a high affinity resin in HAP column while incomplete oligos pass through. Final oligo is produced by removing the protection group of DMT under mild acidic conditions. HAP method provides two major advantages, high purity superior to De-Salted method (>98%), and low cost comparable to PAGE or HPLC methods. Oligos produced by the HAP method has such high purity that they can be directly used for any downstream experiments such as PCR, DNA Sequencing, Gene Synthesis, and Mutagenesis. At present, the most economic method to produce oligos is the De-Salted method, which however, yields products poor in purity. For example, the purity of 20-mer, 40-mer and 60-mer is approximately 68%, 45% and 30% respectively. This is calculated based on the 4 steps in DNA synthesis: De-DMT, Coupling, Oxidation and Capping. The average yield of each cycle is about 98%. The purity of 20-mer is therefore (0.98)20-1 = 68%; of 40-mer = (0.98)40-1 = 45%; and of 60-mer = (0.98)60-1 = 30%. Most laboratories use De-Salted oligos despite of their inferior purity because higher quality oligos produced by alternative methods such as PAGE, HPLC, and OPC are too costly. HAP presents the perfect alternative for high purity oligos at lowest prices. In fact, price is even lower than those of De-Salted method in some cases. |
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PEPTIDE SYTHESIS
Bio Basic Canada Inc. employs fine-tuned synthesis protocols, stringent QA/QC procedures, and state-of-the art instrumentations to assure high-qulaity products are synthesized and delivered.
Key Features
- ISO9001:2000 certified
- Ten years of experience in Peptide Synthesis.
- From small peptides to long peptides ( >100 amino acids)
- From the regular peptides to all kinds of modified peptides
- From mgs to kilograms
- GMP standard peptides are available
- All peptides are delivered with HPLC and Mass Spectroscopy data
- Fast turnaround time: 6-12 working days.
- Low price GUARANTEED
- Worldwide sales network. Bio Basic Canada Inc. has more than 40 worldwide distributors in the world.
Specifications
- All custom peptides are supplied with strict analytical specifications, which include HPLC and MS analysis.
- Most custom peptides can be delivered within 2-3 weeks. The delivery time may vary depending on the peptide length and nature of the sequences, as wel as modifications.
- All information is treated with strict confidentiality. If required, we are pleased to sign a client confidentiality agreement before receiving your compound structure.
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PROTEIN PURIFICATION
Introduction
Bio Basic Canada Inc., an industry leader in gene, peptide synthesis, is pleased to launch the new protein expression and purification service. Bio Basic Canada Inc. has the necessary know-how to deliver the best possible results to our valued customers. We have the capacity to take your project directly from gene synthesis to protein expression, refolding and purification. At Bio Basic Canada Inc., we are proud of our ability to optimize protein expression, maximize product yield, and ensure purity to serve every aspect of your research needs.
From Gene to Protein
Starting from de novo gene synthesis, cDNA or from almost anywhere a client asks us to begin; our scientists can deliver high quality purified protein in a timely manner.
About Protein Purification
Recombinant proteins are used throughout the whole spectrum of biomedical research. Once you have large quantity of purified protein, you could conduct many downstream experiments, such as enzyme kinetics, functional and structural studies.
There are two main systems for the expression of recombinant protein - prokaryotic and eukaryotic.
Prokaryotic recombinant protein expression systems (E.Coli) have several advantages, including ease of culture, rapid cell growth and relatively simple purification procedures. However, most post-translational modifications are not added by bacteria, and most proteins become insoluble in inclusion bodies and are very difficult to recover as functional proteins.
Eukaryotic systems for the expression of protein include yeast, baculovirus cells, and mammalian cells. Advantages of these systems include high levels of expression, no inclusion bodies, and intact post-translational modifications.
Things to Consider
Sequence Optimization: In highly expressed genes, a narrow set of codons is used and these codons correspond to the more abundant tRNA species. This minimizes the risk of tRNA depletion during translation. In fact, the codons in a gene may be true bottlenecks, especially in cases where foreign genes are expressed in a host in which the usage of codons in highly expressed genes does not resemble the usage of codons in the species from which the foreign gene originates. In such cases, it has been shown that substitution of rare codons in the introduced gene may increase the yield dramatically. In addition, replacement of rare codons might decrease the chance of misincorporation and protect the protein from premature turnover (1).
Expression System: In order to decide which host is most suitable the amount and the degree of purity of the product as well as its biological integrity and potential toxicity should be considered. For example, bacterial expression systems are not suitable if post-translational modification is required to produce a fully functional recombinant product (2).
Expression Vector: Once the host has been selected, many different vectors are available for consideration. However, as for the selection of a suitable host system, the final choice of vector should take into consideration the specific requirements of the application and will, of course, be influenced by the behaviour of the target protein.
Tag: As for the selection of host and vectors, the decision to use which tag must be made according to the needs of the specific application. The two most commonly used tags are glutathione S-transferase (GST tag) and 6 x histidine residues ((His)6 tag).
Upon receiving of sequence information, our scientists would analyze the sequence to determine characteristics like molecular weight, pI, solubility, susceptibility to oxidation and hydrophobicity. Every protein is different, there is no standard way for protein expression and purification, and the protocols and strategies must be worked out for each individual protein and with its intended use in mind. Scientists at Bio Basic Canada Inc. use their accumulated extensive working knowledge of purifying recombinant protein to best tailor our service to serve your research needs. At Bio Basic Canada Inc., we offer all four expression systems* to give you the maximum flexibility.
HUMAN GENEPOOL SEARCH
Launching Human GENEPOOLTM Collection
Effective January 1, 2011, Bio Basic Canada Inc, proudly announces the successful launch of Human GENEPOOLTM collection. With a click of a button, researchers may readily place an order for any clone(s) of interest from Bio Basic Canada Inc.’s prestigious Human GENEPOOLTM Collection at lowest price per bp.
High Quality
Bio Basic Canada Inc. has over 20,000 human ORF clones available from our Human GENEPOOLTM collection. Bio Basic Canada Inc.’s Human GENEPOOLTM consists of most commonly cited human ORF cDNA clones and therefore represents the most valuable cDNA collection.
Commitment
Bio Basic Canada Inc. guarantees the ORF clones in Human GENEPOOLTM 100% match the GenBank sequence information. Most of our cDNA clones in the Human GENEPOOLTM collection are obtained directly from our high quality plasmid cDNA libraries, thereby free of any errors from other alternative cloning methods. All PCR-generated clones are fully sequenced and verified to ensure authenticity of the gene. Bio Basic Canada Inc.’s mission is to provide biological and biomedical researchers with cost effective technologies and services at the highest possible quality.
Full Service
When a gene is not available from Bio Basic Canada Inc’s Human GENEPOOLTM collection, Bio Basic Canada Inc, is pleased to offer most competitive price for gene synthesis service per bp |
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www.biobasic.com
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